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Introduction Since the first immunoassays were developed in the 1950's the variety of assay formats has increased dramatically.
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This new method, termed immuno-polymerase chain reaction (immuno-PCR), benefits from the specificity of antibodies and the sensitivity of PCR. Several independent studies undertaken since the publication of this seminal paper have consistently demonstrated the advantages of this technique over traditional ELISA, some achieving more than a 50,000 fold increase in sensitivity over the equivalent immunoassay [2]. Furthermore, immuno-PCR can be used in a multiplex format and offers accurate quantitation.