'auxin', 'grafting' in Plant hormones (Literature sources on phytohormones and plant signalling)

Tags
Current selected tags: 'auxin', 'grafting'. Clear

Your new post is loading...

Scooped by Julio Retamales

Scoop.it!

A PHYB-PIF4-auxin module promotes tomato graft formation in response to elevated ambient temperature

From doi.org - December 30, 2023 2:06 PM

Authors: Xianmin Meng, Feng Zhang, Wencai Yang and Qingmao Shang.

Horticultural Plant Journal (2024)

Abstract: "Grafting is an effective technique for increasing the resistance of vegetables to biotic and abiotic stresses. It has been widely applied to produce solanaceous and melon vegetables. Temperature is an important external factor affecting graft formation. However, the molecular mechanism by which external ambient temperature affects tomato graft formation remains unclear. In this study, we demonstrated that elevating ambient temperature during grafting to 35 °C for more than 24 h after grafting accelerated vascular reconnection. We generated self- or heterografted combinations between phyB1B2 and pif4 loss-of-function mutant and wild-type plants, and were mutants unresponsive to graft formation at elevated ambient temperature. In addition, elevated ambient temperature induced SlPIF4 expression during grafting. SlPIF4 directly binds the promoters of auxin biosynthesis genes SlYUCCAs and activates their expression. Further investigation revealed auxin accumulation in the graft junction under elevated ambient temperature. The results illuminate the mechanism by which the PHYB-PIF4-auxin module promotes tomato graft formation in response to elevated ambient temperature."

more...

No comment yet.

Scooped by Julio Retamales

Scoop.it!

High temperature perception in leaves promotes vascular regeneration and graft formation in distant tissues

From journals.biologists.com - March 1, 2022 9:29 AM

Authors: Phanu T. Serivichyaswat, Kai Bartusch, Martina Leso, Constance Musseau, Akira Iwase, Yu Chen, Keiko Sugimoto, Marcel Quint and Charles W. Melnyk.

Development (2022)

One-sentence summary: This paper describes a pathway in which high temperatures are perceived by the leaves to promote an auxin-dependent signal that accelerates vascular regeneration, graft formation and plant parasitism in distant tissues.

Abstract: "Cellular regeneration in response to wounding is fundamental to maintain tissue integrity. Various internal factors including hormones and transcription factors mediate healing, but little is known about the role of external factors. To understand how the environment affects regeneration, we investigated the effects of temperature upon the horticulturally relevant process of plant grafting. We found that elevated temperatures accelerated vascular regeneration in Arabidopsis thaliana and tomato grafts. Leaves were crucial for this effect, as blocking auxin transport or mutating PHYTOCHROME INTERACTING FACTOR 4 (PIF4) or YUCCA2/5/8/9 in the cotyledons abolished the temperature enhancement. However, these perturbations did not affect grafting at ambient temperatures, and temperature enhancement of callus formation and tissue adhesion did not require PIF4, suggesting leaf-derived auxin specifically enhanced vascular regeneration in response to elevated temperatures. We also found that elevated temperatures accelerated the formation of inter-plant vascular connections between the parasitic plant Phtheirospermum japonicum and host Arabidopsis, and this effect required shoot-derived auxin from the parasite. Taken together, our results identify a pathway whereby local temperature perception mediates long distance auxin signaling to modify regeneration, grafting and parasitism."

Julio Retamales's insight:

An earlier version of this relevant article was already posted here when published as a preprint.

more...

No comment yet.

Scooped by Julio Retamales

Scoop.it!

Cell-wall damage activates DOF transcription factors to promote wound healing and tissue regeneration in Arabidopsis thaliana

From linkinghub.elsevier.com - March 22, 2022 9:28 PM

Authors: Ai Zhang, Keita Matsuoka, Abdul Kareem, Madalen Robert, Pawel Roszak, Bernhard Blob, Anchal Bisht, Lieven De Veylder, Cătălin Voiniciuc, Masashi Asahina and Charles W. Melnyk.

Current Biology (2022)

Editor's view: Plants require an efficient regeneration system to heal wounds. Zhang et al. identify four DOF transcription factors that are rapidly activated at sites of wounding, graft formation, or tissue cutting. These DOFs are activated by cell-wall damage and auxin to promote tissue regeneration and wound healing.

Highlights: • Four DOF transcription factors are rapidly activated at wounding and grafting sites • Damage to or modifications of the cellulose and pectin matrix activate DOFs • DOFs regulate callus formation, tissue adhesion, and vascular regeneration at wounds • Wound-associated ERF115 and ANAC096 are also activated upon cell-wall damage

Abstract: "Wound healing is a fundamental property of plants and animals that requires recognition of cellular damage to initiate regeneration. In plants, wounding activates a defense response via the production of jasmonic acid and a regeneration response via the hormone auxin and several ethylene response factor (ERF) and NAC domain-containing protein (ANAC) transcription factors. To better understand how plants recognize damage and initiate healing, we searched for factors upregulated during the horticulturally relevant process of plant grafting and found four related DNA binding with one finger (DOF) transcription factors, HIGH CAMBIAL ACTIVITY2 (HCA2), TARGET OF MONOPTEROS6 (TMO6), DOF2.1, and DOF6, whose expression rapidly activated at the Arabidopsis graft junction. Grafting or wounding a quadruple hca2, tmo6, dof2.1, dof6 mutant inhibited vascular and cell-wall-related gene expression. Furthermore, the quadruple dof mutant reduced callus formation, tissue attachment, vascular regeneration, and pectin methylesterification in response to wounding. We also found that activation of DOF gene expression after wounding required auxin, but hormone treatment alone was insufficient for their induction. However, modifying cell walls by enzymatic digestion of cellulose or pectin greatly enhanced TMO6 and HCA2 expression, whereas genetic modifications to the pectin or cellulose matrix using the PECTIN METHYLESTERASE INHIBITOR5 overexpression line or korrigan1 mutant altered TMO6 and HCA2 expression. Changes to the cellulose or pectin matrix were also sufficient to activate the wound-associated ERF115 and ANAC096 transcription factors, suggesting that cell-wall damage represents a common mechanism for wound perception and the promotion of tissue regeneration."

more...

No comment yet.