Genetic Engineering Publications - GEG Tech top picks

Here, the authors evaluated the cleavage activities of 218 sgRNAs and found that nucleotides at both PAM-distal and PAM-proximal regions of the sgRNA are significantly correlated with on-target efficiency. They also demonstrated that the genomic context of the targeted DNA, the GC percentage, and the secondary structure of sgRNA are critical factors contributing to cleavage efficiency. This study reveals important parameters for the design of sgRNAs with high on-target efficiencies, especially in the context of high throughput applications.