Genetic Engineering Publications - GEG Tech top picks

In this study, the scientists have developed a powerful new technique to investigate Bcl-2-mediated regulation of mitochondrial outer membrane permeabilization (MOMP)  This method, called mito-priming, uses co-expression of pro- and anti-apoptotic Bcl-2 proteins to engineer Bcl-2 addiction. By combining different pro- and anti-apoptotic Bcl-2 pairings together with CRISPR/Cas9-based genome editing, they find that tBID and PUMA can preferentially kill in a BAK-dependent manner. In summary, mito-priming represents a facile and robust means to trigger mitochondrial apoptosis.