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Genetic Engineering Publications - GEG Tech top picks
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BigField GEG Tech's insight:
In this study, the authors show that a site specific double strand break (DSB) generated both in the genome and the donor plasmid using the CRISPR-Cas9 system can be efficiently used to target ∼5 kb plasmids into mammalian genomes via nonhomologous end joining (NHEJ). They were able to achieve efficiencies of up to 0.17% in HEK293 cells and 0.45% in CHO cells. 
Dominique Blanchard's comment,
May 13, 2015 8:46 AM
I'm going to read this paper because It will greatly impact owners of homologous recombination related patents.
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Scoop.it!
BigField GEG Tech's insight:
Chinese hamster ovary (CHO) cells are the most widely used production host for therapeutic proteins. With the recent emergence of CHO genome sequences, CHO cell line engineering has taken on a new aspect through targeted genome editing.
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In this work, the scientists used CRISPR/Cas9 editing to modify CHO cells to enforce high mTORC1 activity by knocking-out TSC2, a major mTOR inhibitory protein, or PTEN, a phosphatase that attenuates the PI3K/AKT/mTOR pathway. These data underscore manipulation of TSC as a strategy to improve performance of CHO cell in bioreactors.