Genetic Engineering Publications - GEG Tech top picks

In this study, the authors present a high-throughput approach that uses CRISPR interference (CRISPRi) to discover regulatory elements and identify their target genes. They assess >1 megabase (Mb) of sequence in the vicinity of 2 essential transcription factors, MYC and GATA1, and identify 9 distal enhancers that control gene expression and cellular proliferation. This CRISPRi-based approach can be applied to dissect transcriptional networks and interpret the contributions of noncoding genetic variation to human disease.