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Genetic Engineering Publications - GEG Tech top picks

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CRISPR-STAT: an easy and reliable PCR-based method to evaluate target-specific sgRNA activity | Genetic Engineering Publications - GEG Tech top picks | Scoop.it

From nar.oxfordjournals.org - August 25, 2015 7:36 AM

BigField GEG Tech's insight:

In this sutdy, the authors describe an easy, quick and cost-effective fluorescent polymerase chain reaction (PCR)-based method, CRISPR Somatic Tissue Activity Test (CRISPR-STAT), to determine target-specific efficiency of sgRNA. They validated the method using CRISPR/Cas9 and zebrafish which could be applied to other model systems and other genome targeting nucleases.

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CRISPR-STAT: an easy and reliable PCR-based method to evaluate target-specific sgRNA activity