Genetic Engineering Publications - GEG Tech top picks

Forward genetic screens using Sleeping Beauty (SB) mobilized T2/Onc transposons have been used to identify Common Insertion Sites (CIS) associated with tumor formation. Here the authors  developed an automated method to systematically identify T2/Onc-genome RNA fusion sequences in RNA-seq data. RNA fusion based CIS were identified corresponding to both DNA based CIS (Cdkn2a, Mycl1, Nf2, Pten, Sema6d and Rere) and additional regions strongly associated with cancer that were not observed by LM-PCR (Myc, Akt1, Pth, Csf1r, Fgfr2, Wisp1, Map3k5 and Map4k3). These methods independently identify CIS regions, and also point to cancer-associated genes like Braf. They anticipate RNA-seq analyses of tumors from forward genetic screens will become an efficient tool to identify causal events.

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