The authors describe a protocol for the targeted integration of a doxycycline-inducible transgene expression system in a safe harbor site in iPSCs. This gene targeting strategy uses zinc finger nucleases (ZFNs) to enhance homologous recombination at the AAVS. This protocol could be also compatible with the use of TALEN or CRISPR.
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The authors describe a protocol for the targeted integration of a doxycycline-inducible transgene expression system in a safe harbor site in iPSCs. This gene targeting strategy uses zinc finger nucleases (ZFNs) to enhance homologous recombination at the AAVS. This protocol could be also compatible with the use of TALEN or CRISPR.
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