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In this work, the authors describe a highly efficient strategy to obtain bacterial artificial chromosome (BAC) clones of EBV episomes by utilizing CRISPR/Cas9-mediated strand break of the viral genome and subsequent homology-directed repair.
In this work, the authors describe a highly efficient strategy to obtain bacterial artificial chromosome (BAC) clones of EBV episomes by utilizing CRISPR/Cas9-mediated strand break of the viral genome and subsequent homology-directed repair.