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A tool for genome-wide design of CRISPR guide RNAs reduces off-target effects and facilitates targeting of the non-coding genome.
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Here, scientists present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of guide RNAs (gRNAs) for single- and paired-gRNA genome-wide screens. They also show that the trie data structure of GuideScan enables the design of gRNAs that are more specific than those designed by existing tools.