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Genetic Engineering Publications - GEG Tech top picks
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In this study, the authors used Sleeping beauty to mediate transposition of hematopoietic progenitors with a transposon encoding a L22Y variant dihydrofolate reductase (DHFR) fused to GFP. Culture of nucleofected cells in medium under selective conditions increased the frequency of GFP+ drug-resistant hematopoietic colony forming cells by 2-fold.
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