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Genetic Engineering Publications - GEG Tech top picks
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The authors demonstrated that suppressed the NHEJ key molecules such as KU70 or DNA ligase IV promotes the efficiency of HDR 4–5-fold. When co-expressed with the Cas9 system, E1B55K and E4orf6 improved the efficiency of HDR up to eightfold and essentially abolished NHEJ activity in both human and mouse cell lines.
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