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Genetic Engineering Publications - GEG Tech top picks
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Scoop.it!
BigField GEG Tech's insight:
Lentiviral vectors and CRISPR to serve a large scale screening of loss-of-function . Nice!
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Scoop.it!
BigField GEG Tech's insight:
Injection of preneoplastic human mammary epithelial cells into the mammary ducts of immunodeficient mice leads to replacement of the murine luminal layer with morphologically normal human cells. Genetic manipulation of the injected cells by lentiviral vectors makes it possible to study defined steps in the transformation of human mammary epithelial cells in a more physiological environment than has hitherto been possible.
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Here, the authors discuss advantages and challenges of intracellular production strategies and describe emerging approaches based on the direct delivery of protein either by transfer of recombinant protein or by lentiviral protein transduction. With focus on adapting viruses for protein delivery, they describe the concept of ‘all-in-one’ lentiviral particles engineered to co-deliver effector proteins and donor sequences for DNA transposition or homologous recombination.
www.geg-tech.com/Vectors