Genetic Engineering Publications - GEG Tech top picks

Madin-Darby canine kidney II cells are commonly used models to study drug transport. In these cells, however, endogenous transporters such as canine Mdr1/P-glycoprotein (Abcb1) complicate the interpretation of transport studies. 

Here the authors used the CRISPR cas system toknock out endogenous canine Mdr1 and establish a Madin-Darby canine kidney II. They obtained a cell clone with Abcb1 gene alterations and without any cMdr1 expression. Functional studies of these cells, using digoxin and other prototypic MDR1 substrates, showed close to identical transport in the apical-to-basolateral and basolateral-to-apical directions, resulting in efflux ratios indistinguishable from unity.