Genetic Engineering Publications - GEG Tech top picks

Here, the authors used the CRISPR Cas9 system to generate  NPC1-deficient ldl-D cells. They observed that, in the absence of galactose supplementation, NPC1-deficient ldl-D cells also transported more cholesterol from lysosomes to the endoplasmic reticulum, as monitored by an increase in cholesteryl [14C]-oleate levels. These experiments support a model in which NPC1 protein functions to transfer cholesterol past a lysosomal glycocalyx.

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