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PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world.
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The authors describe the generation of PrP knockout clones from epithelial NMuMG cell line using CRISPR-Cas9 knockout technology. The quantitative global proteome analysis demonstrates that ~120 proteins were shown to reproducibly correlate with the presence or absence of PrP, with most of these proteins belonging to extracellular components, cell junctions or the cytoskeleton.
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