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Genetic Engineering Publications - GEG Tech top picks
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Neutralizing the RNA phosphodiester backbone enables delivery of siRNA across cell membranes.
BigField GEG Tech's insight:
The authors design the synthesis of short interfering ribonucleic neutrals (siRNNs) whose phosphate backbone contains neutral phosphotriester groups, allowing for delivery into cells. Once inside cells, siRNNs are converted by cytoplasmic thioesterases into native, charged phosphodiester-backbone siRNAs, which induce robust RNAi responses. geg-tech.com 
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In such fungi, RNAi has been induced by expressing hairpin RNAs delivered through plasmids, sequences integrated in fungal or plant genomes, or by RNAi generated in planta by a plant virus… ...
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BigField GEG Tech's insight:
A new way to avoid genotoxicity? Several types of CRISPR–Cas adaptive immune system have been described, each with a unique mechanism and protein composition. DNA cleavage by type III-A CRISPR–Cas systems relies on a complex known as Cas10–Csm and requires transcription of the target DNA. Type III-A CRISPR–Cas systems are also capable of degrading RNA, although why they should have an RNase function is not known. Jiang et al. now show that some phage targets can delay the transcription-dependent DNA cleavage mechanism of type III-A CRISPR–Cas systems but that CRISPR RNases prevent the replication of these phages.
Martina Hrabinová's curator insight,
March 31, 2016 5:14 AM
A new way to avoid genotoxicity? Several types of CRISPR–Cas adaptive immune system have been described, each with a unique mechanism and protein composition. DNA cleavage by type III-A CRISPR–Cas systems relies on a complex known as Cas10–Csm and requires transcription of the target DNA. Type III-A CRISPR–Cas systems are also capable of degrading RNA, although why they should have an RNase function is not known. Jiang et al. now show that some phage targets can delay the transcription-dependent DNA cleavage mechanism of type III-A CRISPR–Cas systems but that CRISPR RNases prevent the replication of these phages.
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PLOS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. Reports of well-performed scientific studies from all disciplines freely available to the whole world.
BigField GEG Tech's insight:
Thanks to RNAi, the authors showed that HPP-4382 can inhibit Bach1 activity in a reporter assay that measures transcription driven by the human HMOX1 E2 enhancer. These results suggest that HPP-4382 is a novel activator of the antioxidant response through the modulation of Bach1 binding to the ARE binding site of target genes.
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Challenges in the delivery of small interfering RNA (siRNA) have hampered clinical translation. Polymeric or periodic short hairpin RNAs (p-shRNAs) are a recent development that can potentially address these delivery barriers by showing improved stability and complexation to enable nanoparticle packaging. Here The scientists modify these biomacromolecules packaging via structural and sequence engineering coupled with selective enzymatic digestion to generate an open-ended p-shRNA (op-shRNA) that is cleaved over ten times more efficiently to yield siRNA. The op-shRNA induces considerably greater gene silencing than p-shRNA in multiple cancer cell lines up to nine days. Op-shRNA provides an RNAi platform that can potentially be packaged and delivered efficiently to disease sites with higher therapeutic efficacy.