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Genetic Engineering Publications - GEG Tech top picks
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BigField GEG Tech's insight:
In this review, the authors give their definition of the concept of synthetic epigenetics and outline the available genome targeting technologies, which are used for locus-specific editing of epigenetic signals. They report early success stories and the lessons they have learned from them, and provide a guide for their application. Finally, they discuss existing limitations of the available technologies and indicate possible areas for further development. 
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BigField GEG Tech's insight:
The authors analyzed the genome-wide effects of TALE- and CRISPR-based transcriptional activators in human cells. Their results show that these transcription factors are highly specific in both DNA-binding and gene regulation, and are able to open targeted regions of closed chromatin independent of gene activation. Collectively, these results underscore the potential for these technologies to make precise changes to gene expression for gene and cell therapies or fundamental studies of gene function.
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The authors explore TALE–DNA interactions to crevasse off target activity. They find that protein context features exert significant influences on binding and thus the canonical recognition code does not fully capture the complexity of TALE–DNA binding.They provide Specificity Inference For TAL-Effector Design (SIFTED) as a publicly available web tool that predicts potential genomic off-target sites for improved TALE design.
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