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Genetic Engineering Publications - GEG Tech top picks

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A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression | Genetic Engineering Publications - GEG Tech top picks | Scoop.it

From www.genomebiology.com - November 17, 2015 2:55 AM

BigField GEG Tech's insight:

To overcome the low CRISPR-Cas9 editing efficiency at the TERT locus, the scientists develop a two-step “pop-in/pop-out” strategy to enrich cells that underwent homologous recombination (HR). This method provides a useful tool for studying telomerase biology, and suggests a general approach to edit loci with low targeting efficiency and to purify and visualize low abundance proteins.

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A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression