Genetic Engineering Publications - GEG Tech top picks

The authors identify rules for specific targeting of transcriptional repressors (CRISPRi), and activators (CRISPRa) to endogenous genes via endonuclease-deficient Cas9. Using these rules, they construct genome-scale CRISPRi and CRISPRa libraries. Growth-based screens identify essential genes, tumor suppressors, and regulators of differentiation. These results establish CRISPRi and CRISPRa as powerful tools that provide information for mapping complex pathways.

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