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Genetic Engineering Publications - GEG Tech top picks
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The repair of a point mutation can be facilitated by combined activity of a single-stranded oligonucleotide and a CRISPR/Cas9 system.
BigField GEG Tech's insight:
The repair of a point mutation can be facilitated by combined activity of a single-stranded oligonucleotide and a CRISPR/Cas9 system. In this study, the scientists provide a detailed analysis of allelic variance at and surrounding the target site. In one particular case, they report sequence alteration directed by a distinct member of the same gene family. Their data suggests that single-stranded DNA molecules may influence DNA junction heterogeneity created by CRISPR/Cas9. 
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evaluated whether CRISPR-Cas9-mediated insertion/deletion (indel) mutagenesis can produce drug-resistance alleles at endogenous loci. This method takes advantage of the heterogeneous in-frame alleles produced following Cas9-mediated DNA cleavage, which we show can generate rare alleles that confer resistance to the growth-arrest caused by chemical inhibitors.