![Long-term and efficient expression of human [beta]-globin gene in a hematopoietic cell line using a new site-specific integrating non-viral system | Genetic Engineering Publications - GEG Tech top picks | Scoop.it](https://img.scoop.it/McIgc7_5fF4eSerUlFxk4jl72eJkfbmt4t8yenImKBVvK0kTmF0xjctABnaLJIm9)
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Genetic Engineering Publications - GEG Tech top picks
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The authors used phiC31 integrase to insert a copy of a β-globin / puromycin (purr) vector into specific genomic locations of a human hematopoietic cell line. After genomic integration, they used Cre recombinase to remove the bacterial backbone and purr.Following that, the stable β-chain expression was continued for several months in the absence of selective pressure.
www.geg-tech.com/Vectors