Based on CRISPR/Cas9 assisted homologous recombination, the authors demonstrated that clean markerless gene deletion from the chromosome can be easily achieved with a high efficiency through a single-step transformation in Clostridium beijerinckii NCIMB 8052, one of the most prominent strains for acetone, butanol and ethanol production.
A series of detailed genetic studies points scientists to a new way to dramatically increase the accumulation of oil in plant leaves, an abundant source of biomass for fuel production.
The scientists find that overexpression of several genes improves the E. coli's tolerance to exogenously added isopentenol. In this way, they improved the production of isopentenol in E. coli, with the methionine biosynthesis regulator MetR improving the titer for isopentenol production by 55%.
Researchers sequence the entire genome of the Clostridium autoethanogenum bacterium, which is used to sustainably produce fuel and chemicals from a range of raw materials, including gases derived from biomass and industrial wastes.
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Researchers sequence the entire genome of the Clostridium autoethanogenum bacterium, which is used to sustainably produce fuel and chemicals from a range of raw materials, including gases derived from biomass and industrial wastes.
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Based on CRISPR/Cas9 assisted homologous recombination, the authors demonstrated that clean markerless gene deletion from the chromosome can be easily achieved with a high efficiency through a single-step transformation in Clostridium beijerinckii NCIMB 8052, one of the most prominent strains for acetone, butanol and ethanol production.
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