Genetic Engineering Publications - GEG Tech top picks
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'We could make that!' — chance meeting leads to creation of antibiotic spider silk - 

'We could make that!' — chance meeting leads to creation of antibiotic spider silk -  | Genetic Engineering Publications - GEG Tech top picks | Scoop.it
Antibiotic 'spider silk' - 'click-chemistry' can be used to attach molecules, such as antibiotics or fluorescent dyes, to artificially produced spider silk synthesised by E.coli bacteria.
BigField GEG Tech's insight:

After five years' work an interdisciplinary team of scientists at The University of Nottingham has developed a technique to produce chemically functionalised spider silk that can be tailored to applications used in drug delivery, regenerative medicine and wound healing.

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CRISPR-Cas targeted plasmid integration into mammalian cells via non-homologous end joining

CRISPR-Cas targeted plasmid integration into mammalian cells via non-homologous end joining | Genetic Engineering Publications - GEG Tech top picks | Scoop.it
BigField GEG Tech's insight:

In this study, the authors show that a site specific double strand break (DSB) generated both in the genome and the donor plasmid using the CRISPR-Cas9 system can be efficiently used to target ∼5 kb plasmids into mammalian genomes via nonhomologous end joining (NHEJ). They were able to achieve efficiencies of up to 0.17% in HEK293 cells and 0.45% in CHO cells.


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Dominique Blanchard's comment, May 13, 2015 8:46 AM
I'm going to read this paper because It will greatly impact owners of homologous recombination related patents.