Genetic Engineering Publications - GEG Tech top picks

Here, the scientists report on the structures of Cas1-Cas2-dual-forked DNA complexes in an effort toward understanding how the protospacer is sampled prior to insertion into the CRISPR locus. Their study provides important structure-based mechanistic insights into PAM-dependent spacer acquisition.

www.geg-tech.com/Vectors

Here, the scientists use single-molecule imaging to visualize Cascade and Cas3 binding to foreign DNA targets. Their analysis reveals two distinct pathways dictated by the presence or absence of a protospacer-adjacent motif (PAM). Binding to a protospacer flanked by a PAM recruits a nuclease-active Cas3 for degradation of short single-stranded regions of target DNA, whereas PAM mutations elicit an alternative pathway that recruits a nuclease-inactive Cas3 through a mechanism that is dependent on the Cas1 and Cas2 proteins. 

www.geg-tech.com/Vectors