To efficiently enrich genetically modified cells, the authors developed two dual-reporter surrogate systems, namely NHEJ-RPG and SSA-RPG based on NHEJ and SSA repair mechanisms, respectively. By utilizing the SSA-RPG reporter, they achieved the enrichment for indels in several endogenous loci with 6.3- to 34.8-fold of non-selected cells.
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To efficiently enrich genetically modified cells, the authors developed two dual-reporter surrogate systems, namely NHEJ-RPG and SSA-RPG based on NHEJ and SSA repair mechanisms, respectively. By utilizing the SSA-RPG reporter, they achieved the enrichment for indels in several endogenous loci with 6.3- to 34.8-fold of non-selected cells.
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