Genetic Engineering Publications - GEG Tech top picks

To extend the catalog of human core and context-dependent fitness genes, the authors of the study have developed a high-complexity second-generation genome-scale CRISPR-Cas9 gRNA library and applied it to fitness screens in five human cell lines. Theypresent a list of 1,580 human core fitness genes and describe their general properties. The rigorous identification of human cell line fitness genes using a high-complexity CRISPR-Cas9 library affords a high-resolution view of the genetic vulnerabilities of a cell.

In this study, the authors developed a high-complexity second-generation genome-scale CRISPR-Cas9 gRNA library and applied it to fitness screens in five human cell lines. Using an improved Bayesian analytical approach, they consistently discover 5-fold more fitness genes than were previously observed. The rigorous identification of human cell line fitness genes using a high-complexity CRISPR-Cas9 library affords a high-resolution view of the genetic vulnerabilities of a cell.

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