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Genetic Engineering Publications - GEG Tech top picks
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Scoop.it!
BigField GEG Tech's insight:
In this study, the authors show that a site specific double strand break (DSB) generated both in the genome and the donor plasmid using the CRISPR-Cas9 system can be efficiently used to target ∼5 kb plasmids into mammalian genomes via nonhomologous end joining (NHEJ). They were able to achieve efficiencies of up to 0.17% in HEK293 cells and 0.45% in CHO cells. 
Dominique Blanchard's comment,
May 13, 2015 8:46 AM
I'm going to read this paper because It will greatly impact owners of homologous recombination related patents.
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The scientists use CRISPR/Cas9-mediated genome editing to demonstrate discrete and opposing roles for the lncRNA Haunt transcript and DNA at the HOXA locus during ESC differentiation.
www.geg-tech.com/Vectors