Genetic Engineering Publications - GEG Tech top picks

In this study the authors have identified small molecules (such as L755507, Brefeldin A, AZT or TFT) that can enhance CRISPR-mediated HDR efficiency, 3-fold for large fragment insertions and 9-fold for point mutations. Interestingly, we have also observed that a small molecule that inhibits HDR can enhance frame shift insertion and deletion (indel) mutations mediated by NHEJ. The identified small molecules function robustly in diverse cell types with minimal toxicity.

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