In this work, the scientists describe FusX, a streamlined Golden Gate TALE assembly system that (1) is backward compatible with popular TALE backbones, (2) is functionalized as a single-tube 3-day TALE assembly process (3) requires only commonly used basic molecular biology reagents (4) and is cost-effective. Over 100 TALEN pairs have been successfully assembled using FusX, and 27 pairs were quantitatively tested in zebrafish with each showing high somatic and germline activity.
To get content containing either thought or leadership enter:
To get content containing both thought and leadership enter:
To get content containing the expression thought leadership enter:
You can enter several keywords and you can refine them whenever you want. Our suggestion engine uses more signals but entering a few keywords here will rapidly give you great content to curate.
In this work, the scientists describe FusX, a streamlined Golden Gate TALE assembly system that (1) is backward compatible with popular TALE backbones, (2) is functionalized as a single-tube 3-day TALE assembly process (3) requires only commonly used basic molecular biology reagents (4) and is cost-effective. Over 100 TALEN pairs have been successfully assembled using FusX, and 27 pairs were quantitatively tested in zebrafish with each showing high somatic and germline activity.