In this report, the authors driven the integration of exogenous DNA sequences to the X-linked Hprt gene of mouse embryonic stem cells thanks to the CRISPR system. They show here that a simple fluorescence in situhybridization (FISH)-based strategy allows the detection and the frequency evaluation of non-specific integrations of a given plasmid.
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In this report, the authors driven the integration of exogenous DNA sequences to the X-linked Hprt gene of mouse embryonic stem cells thanks to the CRISPR system. They show here that a simple fluorescence in situhybridization (FISH)-based strategy allows the detection and the frequency evaluation of non-specific integrations of a given plasmid.
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