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Genetic Engineering Publications - GEG Tech top picks
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This work describes a new high-throughput screening platform for the optimization of CRISPR-Cas9 delivery strategies. The results suggest design principles for the development of materials and transfection strategies with lipid-based materials. The presented platform could be applied to rapidly optimize materials for gene-editing in a variety of cell/tissue types in order to advance genomic medicine, regenerative biology and drug discovery. 
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In this work, the authors reported CRISPR/Cas9-mediated editing of the HSV-1 genome in human cells, including the knockout and replacement of large genes. Introducing the NHEJ inhibitor SCR7 to the CRISPR/Cas9 system greatly facilitated HDR-mediated gene replacement in the HSV-1 genome. They provided the first genetic evidence that two copies of the ICP0 gene in different locations on the same HSV-1 genome could be simultaneously modified with high efficiency and with no off-target modifications.