Genetic Engineering Publications - GEG Tech top picks

The scientists describe an optimized stepwise protocol to deliver CRISPR/Cas9 plasmids in human iPS cells. Based on a two-steps clonal isolation protocol (mechanical picking followed by enzymatic dissociation), they succeed to select and expand corrected human iPS cell line with a great efficiency, more than 2 % of the sequenced colonies (and about 15% to obtain KO by NHEJ). 

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