Research and publish the best content.
Get Started for FREE
Sign up with Facebook Sign up with X
I don't have a Facebook or a X account
Already have an account: Login
Genetic Engineering Publications - GEG Tech top picks
36.6K views |
+0 today
 Your new post is loading...
Your new post is loading...
Scoop.it!
Scientific Reports is an online, open access journal from the publishers of Nature. The 2014 Impact Factor for Scientific Reports is 5.578.
BigField GEG Tech's insight:
In this sutdy, the authors investigated luciferase-mediated bioluminescence imaging (BLI) as a non-invasive technique to continuously monitor cellular behavior in ex vivo whole organ culture. Their results suggest that BLI is a promising tool to monitor spatial and temporal cellular behavior in ex vivo organ culture. 
|
Scoop.it!
BigField GEG Tech's insight:
Here the scientists present a live imaging system for targeted detection of genomic regions. Their approach involves the CRISPR/cas9 system and allows for rapid, stable, low-background visualization of target loci. They demonstrate the efficiency and flexibility of this method by simultaneously labelling major and minor satellite regions as well as two individual loci on mouse chromosome 12. This system provides a tool for dual-colour labelling, which is important for tracking the dynamics of chromatin interactions and for validating epigenetic processes identified in fixed cells.
|
Cas9 can be implemented as RNA-targeting system to track mRNA in living cells. Nuclear export is enabled by efficient targeting of GFP-fused Cas9 to an endogenous mRNA. The approach provides a new and versatile platform for RNA-targeting with applications in RNA imaging and beyond.