Genetic Engineering Publications - GEG Tech top picks

In this study, Cis-regulatory genomic regions necessary for gene expression are revealed by a high-throughput CRISPR-Cas9-based assay.

In this study, the authors show that human IDH mutant gliomas exhibit hypermethylation at cohesin and CCCTC-binding factor (CTCF)-binding sites, compromising binding of this methylation-sensitive insulator protein. They used the CRISPR system to disrupt CTCF and observed in IDH wild-type gliomaspheres the upregulation of PDGFRA and the increasing of proliferation