Genetic Engineering Publications - GEG Tech top picks

In this work, the authors used the ΦC31 integrase system to study the effects of the continuous exposure of exogenous sclerostin on bone. They injected Sost-attB plasmid with ΦC31 integrase plasmid into the mouse tail vein using a hydrodynamic-based method. Sclerostin was expressed in the hepatocytes, secreted into the blood flow, and maintained at high concentrations in the mice injected. These mice showed trabecular bone loss on micro-CT analysis. These findings may help to further ascertain the effects of sclerostin introduced exogenously on the skeleton.