Genetic Engineering Publications - GEG Tech top picks

The authors demonstrated that suppressed the NHEJ key molecules such as KU70 or DNA ligase IV promotes the efficiency of HDR 4–5-fold. When co-expressed with the Cas9 system, E1B55K and E4orf6 improved the efficiency of HDR up to eightfold and essentially abolished NHEJ activity in both human and mouse cell lines.

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The authors targeted DNA ligase IV, a key enzyme in the NHEJ pathway, using the inhibitor Scr7 to promote HDR at the expense of NHEJ. Scr7 treatment increased the efficiency of HDR-mediated genome editing, using Cas9 in mammalian cell lines and in mice for all four genes examined, up to 19-fold.

www.geg-tech.com/Vectors