Here, the authors introduce and optimize a microfluidic membrane deformation method to deliver sgRNA and Cas9 into different cell types and achieve successful genome editing, including for hard-to-transfect lymphoma cells and embryonic stem cells, This method could potentially enable new avenues of biomedical research and gene targeting therapy such as mutation correction of disease genes through combination of the CRISPR-Cas9–mediated knockin system.
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identifying CRISPR/Cas-induced mutations via deep sequencing.