Genetic Engineering Publications - GEG Tech top picks

Here the authors report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Their vector design opens for rapid and easy adaption of the construct to the target chosen. To screen for CRISPR/Cas9 mutants they employed high resolution melting based PCR assays, mutants were confirmed by sequencing and further validated by functional analyses.