MicroRNA (miRNA) sponges are RNA transcripts containing multiple high-affinity binding sites that associate with and sequester specific miRNAs to prevent them from interacting with their target messenger (m)RNAs.
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Genetic Engineering Publications - GEG Tech top picks
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Scoop.it!
BigField GEG Tech's insight:
The scientists demonstrate a possible new mechanism by which HDACi influence tumorigenesis and apoptosis via downregulation of miR-125a-5p expression. This study provides clinical implications in cancer chemotherapy using HDACi. 
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Scoop.it!
MicroRNAs are small and non-coding RNA molecules with the master role in regulation of gene expression at post-transcriptional/translational levels.
BigField GEG Tech's insight:
In this study, the scientists clone CRISPR/cas9 constructs with single-guide RNAs specifically targeting biogenesis processing sites of selected microRNAs; and they find that CRISPR/cas9 can robustly and specifically reduce the expression of these microRNAs up to 96%. This is the first demonstration of the long term stability of microRNA knockdown phenotype by CRISPR/cas9 in both in vitro and in vivo models.
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In this study, the scientists introduce microRNA sponge generator and tester (miRNAsong), a freely available web-based tool for generation and in silico testing of miRNA sponges. This tool generates miRNA sponge constructs for specific miRNAs and miRNA families/clusters and tests them for potential binding to miRNAs in selected organisms. Currently, miRNAsong allows for testing of sponge constructs in 219 species covering 35,828 miRNA sequences.