Your new post is loading...
α-synuclein (α-SYN) is a major pathologic contributor to Parkinson’s disease (PD).
|
Scooped by BigField GEG Tech |
Scoop.it!
No comment yet.
Sign up to comment
Here the authors developed a stable cell line expressing α-SYN endogenously tagged with NanoLuc luciferase reporter using CRISPR/Cas9-mediated genome editing. This allows efficient measurement of transcriptional activity of α-SYN in its native epigenetic landscape which is not achievable using exogenous transfection-based luciferase reporter assays. For the authors, this is the first report showing endogenous monitoring of α-SYN transcription, thus making it an efficient drug screening tool that can be used for therapeutic intervention in PD.