Authors introduce their routine workflow for the production of genetically engineered pigs, especially focused on the genetic modification of somatic donor cells, Somatic Cell Nuclear Transfer using in vitro matured oocytes, and laparoscopic embryo transfer.
The authors compare the genetic and epigenetic integrity of human nuclear-transfer embryonic stem cell (NT-ESC) lines and isogenic induced pluripotent stem cell (iPSC) lines. As iPSCs, NT-ESCs displayed clone- and gene-specific aberrations in DNA methylation and allele-specific expression of imprinted genes. The occurrence of these genetic and epigenetic defects in both NT-ESCs and iPSCs suggests that they are inherent to reprogramming, regardless of derivation approach.
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Authors introduce their routine workflow for the production of genetically engineered pigs, especially focused on the genetic modification of somatic donor cells, Somatic Cell Nuclear Transfer using in vitro matured oocytes, and laparoscopic embryo transfer.
geg-tech.com