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Genetic Engineering Publications - GEG Tech top picks
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In this study, the scientists designed a system of piggyBac transposon-mediated vector which contains SV40 T-Ag cassette that can be removed by flippase recognition target (FRT) recombinase. They demonstrated that this system can amplify human DFCs through reversible immortalization. The immortalized DFCs (iDFCs) exhibit higher proliferate activity, which can be reversed to its original level before immortalization when deimmortalized by FLP recombinase.
www.geg-tech.com/Vectors