Here, the auhtors established a new method for generating pig cell lines using the Sleeping Beauty (SB) transposon-mediated ectopic expression of porcine telomerase reverse transcriptase (pTERT). After passage for more than 40 generations, the cell line retained stable expression of ectopic pTERT and continuous growth potential. These data suggest that the new method established is useful for generating pig cell lines without viral sequence and antibiotic resistant gene.
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Here, the auhtors established a new method for generating pig cell lines using the Sleeping Beauty (SB) transposon-mediated ectopic expression of porcine telomerase reverse transcriptase (pTERT). After passage for more than 40 generations, the cell line retained stable expression of ectopic pTERT and continuous growth potential. These data suggest that the new method established is useful for generating pig cell lines without viral sequence and antibiotic resistant gene.
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