The authors present a protocol for using ΦC31 and Bxb1 ntegrases simultaneously to facilitate cassette exchange at a predefined location. This approach permits greater control and accuracy over integration. They also present a general method for using polymerase chain reaction assays to verify that the desired cassette exchange occurred successfully.
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The authors present a protocol for using ΦC31 and Bxb1 ntegrases simultaneously to facilitate cassette exchange at a predefined location. This approach permits greater control and accuracy over integration. They also present a general method for using polymerase chain reaction assays to verify that the desired cassette exchange occurred successfully.
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