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Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering | The guide RNA and donor DNA of the CRISPR/Cas system tolerate large chemical modifications and can be engineered for enhanced delivery and gene editing. 
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In this report, the scientists demonstrate that the gRNAs of Cas9 and Cpf1, and donor DNA can be chemically modified at their terminal positions without losing activity. The tolerance of the gRNA and donor DNA to chemical modifications has the potential to enable new strategies for genome engineering.