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Genetic Engineering Publications - GEG Tech top picks
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Tryout sets are made to make experimental set up easier. They are groups of ready-to-use lentiviral vectors, comparable with each other, allowing the choice ...
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This video shows how to find the best customization of a gene transfer tool according your experimental context. 
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We describe the generation of a set of plasmid vector tools that allow the rapid generation of complex-interacting stable transgenes in immortalized and primary cells.
BigField GEG Tech's insight:
In this work, the scientists describe the generation of a set of plasmid vector tools that allow the rapid generation of complex-interacting stable transgenes in immortalized and primary cells. This system incorporates MultiSite-Gateway cloning for the rapid generation of vectors allowing flexible choice of promoters and transgenes, and Sleeping Beauty transposase technology for efficient incorporation of multiple transgenes in into host cell DNA. The vectors and a library of compatible Gateway Entry clones are available from the non-profit plasmid repository Addgene.
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This introduction video shows how the choice of every vector element can modify the lentiviral vector's activity.
BigField GEG Tech's insight:
Video which explains the influence of promoter, integrase or envelope on the activity of lentiviral vectors. The goal is to design the best vector according to the experimental needs.
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In this study, the authors present a high-throughput approach that uses CRISPR interference (CRISPRi) to discover regulatory elements and identify their target genes. They assess >1 megabase (Mb) of sequence in the vicinity of 2 essential transcription factors, MYC and GATA1, and identify 9 distal enhancers that control gene expression and cellular proliferation. This CRISPRi-based approach can be applied to dissect transcriptional networks and interpret the contributions of noncoding genetic variation to human disease.