This study describes a protocol for using the PB-based system for inducible expression of bovine rhodopsin in HEK293S GnTI− cells. Using this protocol, scientists expressed and purified 26 rhodopsin mutants to be used for site-directed spin labeling.
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This study describes a protocol for using the PB-based system for inducible expression of bovine rhodopsin in HEK293S GnTI− cells. Using this protocol, scientists expressed and purified 26 rhodopsin mutants to be used for site-directed spin labeling.
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