This study demonstrates a knockin strategy without drug selection for both active and silent genes in human embryonic stem cells (hESCs) thanks to the iCRISPR platform. This selection-free knockin strategy is expected to greatly facilitate the use of hESCs for developmental studies, disease modeling, and cell-replacement therapy.
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This study demonstrates a knockin strategy without drug selection for both active and silent genes in human embryonic stem cells (hESCs) thanks to the iCRISPR platform. This selection-free knockin strategy is expected to greatly facilitate the use of hESCs for developmental studies, disease modeling, and cell-replacement therapy.
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